Bio-One Franchise FDD, Profits & Costs (2025)
Bio-One Inc. is a well-established franchise specializing in biohazard cleanup and decontamination services. Founded in 2006, the company is based in Greenwood Village, Colorado, and began offering franchise opportunities in 2010.
With a focus on critical services like crime scene cleanup, hoarding remediation, and biohazard decontamination, Bio-One operates with sensitivity and professionalism, assisting individuals and families in difficult circumstances.
What sets Bio-One apart is its pioneering role in the industry and its unwavering commitment to the philosophy of “Help First, Business Second.” This ensures that franchisees receive thorough training to handle emotionally challenging situations with care, discretion, and a deep sense of responsibility to the communities they serve.
The most profitable franchises to own in 2025
We have compiled the list of the top 400 franchises across 9 industries. Download the report below.
Initial Investment
How much does it cost to start a Bio-One franchise? It costs on average between $116,000 – $170,000 to start a Bio-One franchised facility.
This includes expenses for specialized equipment, protective gear, cleaning supplies, and initial operating costs. The total investment varies based on several factors, such as the service area, the scope of cleanup services offered, and whether the franchisee opts to lease or buy a facility.
| Type of Expenditure | Amount |
|---|---|
| IFF | $60,000 |
| QSP Package | $39,500 |
| Technology Startup Fee | $1,595 |
| Annual Conference Registration Deposit | $1,000 |
| Rent and Rental Improvements | $0 – $600 |
| Furniture, Fixtures, and Equipment | $0 – $2,000 |
| Computer Hardware, Software, and Electronics | $800 – $7,800 |
| Initial Training Expenses | $1,000 – $2,500 |
| OSHA Training Fee | $1,500 |
| OSHA Training Expenses | $1,000 – $2,500 |
| Business Vehicle | $0 – $30,000 |
| Business Vehicle Tax, Title, and License | $0 – $1,500 |
| Business Vehicle Insurance | $150 – $300 |
| Insurance and Professional Services | $750 – $4,000 |
| Additional Funds – 3 months | $9,000 – $15,000 |
| TOTAL | $116,295 – $169,795 |
Average Revenue (AUV)
How much revenue can you make with a Bio-One franchise? A Bio-One franchised location makes on average $423,000 in revenue (AUV) per year.
Here is the extract from the Franchise Disclosure Document:
This compares to $427,000 yearly revenue for similar commercial cleaning franchises. Below are a few Bio-One competitors as a comparison:
The most profitable franchises to own in 2025
We have compiled the list of the top 400 franchises across 9 industries. Download the report below.
Bio-One Franchise Disclosure Document
Frequently Asked Questions
How many Bio-One locations are there?
As of the latest data, Bio-One operates 146 franchised locations across 41 states in the United States. All Bio-One offices are independently owned and operated by local franchisees; the company does not maintain any company-owned locations.
What is the total investment required to open a Bio-One franchise?
The total investment required to open a Bio-One franchise ranges from $116,000 to $170,000.
What are the ongoing fees for a Bio-One franchise?
Bio-One franchisees pay a royalty fee of 7.5% of gross sales or a minimum monthly royalty, starting at $750 in the first year, increasing to $1,500 in the second year, and $2,250 from the third year onward.
They also contribute a marketing fee of 2% of gross sales or a minimum of $200 per month, along with a required local advertising spend of 5% of gross sales or at least $1,500 per month.
What are the financial requirements to become a Bio-One franchisee?
To become a Bio-One franchisee, candidates are generally required to have a minimum net worth ranging from $125,000 to $250,000 and liquid capital between $35,000 and $75,000. These financial thresholds ensure that franchisees possess the necessary resources to effectively establish and operate their Bio-One franchise.
How much can a Bio-One franchise owner expect to earn?
The average gross sales for a Bio-One franchise are approximately $0.43 million per location. Assuming a 15% operating profit margin, $0.43 million yearly revenue can result in $65,000 EBITDA annually.
Who owns Bio-One?
Bio-One franchise is owned by Five Star Franchising, an innovative platform of home service brands.
Disclaimer
Disclaimer: This content has been made for informational and educational purposes only. We do not make any representation or warranties with respect to the accuracy, applicability, fitness, or completeness of the information presented in the article. You should not construe any such information or other material as legal, tax, investment, financial, or other professional advice. Nothing contained in this article constitutes a solicitation, recommendation, endorsement, advertisement, or offer to buy or sell any franchises, securities, or other financial instruments in this or in any other jurisdiction in which such solicitation or offer would be unlawful under the franchise and/or securities laws of such jurisdiction.
All content in this article is information of a general nature and does not address the detailed circumstances of any particular individual or entity. Nothing in the article constitutes professional and/or financial and/or legal advice, nor does any information in the article constitute a comprehensive or complete statement of the matters discussed or the law relating thereto. You alone assume the sole responsibility of evaluating the merits and risks associated with the use of any information or other content in this article before making any decisions based on such information or other content.
